Production of in vivo biotinylated scFv specific to almond ( Prunus dulcis ) proteins by recombinant Pichia pastoris
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چکیده
منابع مشابه
Production of Recombinant Human Granulocyte-Colony Stimulating Factor by Pichia pastoris
Human granulocyte-colony stimulating factor (hG-CSF) cDNA was expressed in the methylotrophic yeast Pichia pastoris under the control of the alcohol oxidase (AOX1) promoter. An expression vector for hG-CSF secretion was constructed using vector pPIC9. Higher levels of hG-CSF was obtained using a P. pastoris Mut+ (methanol utilization fast) phenotype. The effects of environmental factors such as...
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Pichiapastoris is one of the most widely used expression systems for the production of recombinant secretory proteins. Its universal application is, however, somewhat hampered by its unpredictable yields for different heterologous proteins, which is now believed to be caused in part by their varied efficiencies to traffic through the host secretion machinery. The yeast endoprotease Kex2 removes...
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LEVEL: INTERMEDIATE Ever since Invitrogen of Carlsbad, CA (www.invitrogen.com) acquired the distribution rights in 1993 for the Pichia pastoris yeast protein expression system, P. pastoris has gained widespread popularity, as witnessed by an increasing number of publications (Figure 1). The expression system was originally developed as a single-cell protein production platform by Philips Petrol...
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Background: The methylotrophic yeast Pichia pastoris is an appealing production host for a variety of recombinant, including biologics. In this sense, various genetic- and non-genetic-based techniques have been implemented to improve the production efficiency of this expression platform. Los1 (loss of supression) encodes a non-essential nuclear tRNA exporter in Saccharomyces cerevisiae, which i...
متن کاملExpression of recombinant proteins in the methylotrophic yeast Pichia pastoris.
Protein expression in the microbial eukaryotic host Pichia pastoris offers the possibility to generate high amounts of recombinant protein in a fast and easy to use expression system. As a single-celled microorganism P. pastoris is easy to manipulate and grows rapidly on inexpensive media at high cell densities. Being a eukaryote, P. pastoris is able to perform many of the post-translational mo...
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ژورنال
عنوان ژورنال: Journal of Biotechnology
سال: 2016
ISSN: 0168-1656
DOI: 10.1016/j.jbiotec.2016.04.024